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A NTA flow cell was run in parallel in the same conditions as a blank.
The flow cell was run for 6 hours for each sequencing run.
The Illumina GAIIx flow cell was run for 75 cycles using a single-read recipe (v4 sequencing kits) according to the manufacturer's instructions.
The cell was run for varying times, Vancomycin concentrations and voltages, to gain information on optimisation of conditions for impregnating the graft.
The flow cell was run for 76 cycles of sequencing on an Illumina GAIIx sequencer with an additional six cycles for the indexing.
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For the enrichment without COT-1 one lane of Illumina flow-cell was run, and for the experiment with COT-1, two such lanes were run.
A control experiment with mock cells was run as part of each flow cytometer experiment to determine where the cut-off line should fall.
A culture medium sample corresponding to 1.5×106 cells was run in polyacrylamide gel electrophoresis and transferred to an Immobilon-P Transfer Membrane (Millipore).
Protein extract from these cells was run on a gel to confirm complete suppression of p53 as described earlier [ 34].
Cell lysate and conditioned medium from 4T1.2 cells was run alongside these samples to allow size alignment (lanes 1 and 2, respectively).
All the four EIX cells were run for a maximum of 7 h.
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