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Finally, an in-line measurement cell was added to the set-up to allow continuous measurement of the turbidity.
The desired ratio of internal standard molecules per cell was added.
After 24 h of incubation, cells were washed with PBS and 1 ml of fresh medium containing 5 adenovirus pfu/plated cell was added to each well.
An appropriate volume for a final infection of 50 bacteria per host cell was added to the apical or basolateral compartment, as indicated, for various times.
After 24 h incubation, cells were washed with PBS and 1 ml of fresh medium containing 5 adenovirus molecules of infection (MOI or pfu /plated) per cell was added to each well [25].
Further, it was demonstrated that when one lung cancer cell (A549 cell) was added into 3 ml peripheral blood, LunX mRNA could be detectable as a positive case in our established method (data not shown).
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CM from microglial cells was added to cultures of NPCs and cell viability determined as below.
Conditioned medium (1 ml) from the supernatant of each 786-O stable cells was added, respectively.
Then, 100 µL of HMSC suspension (105 cells) was added to the upper chamber.
Fifty µl (2.5×104 cells) was added to each well of a 96-well v-shaped plate.
After incubation of the serum/virus mixtures, a suspension of BSR cells was added.
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