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The vital stain methylene blue was used for a qualitative assessment of cell vitality.
Cell vitality was verified 24 h upon encapsulation.
We observed augmented cell vitality in CD80/4-1BBL-T-cells CD80/4-1BBL-T-cells CD80/4-1BBL-T-cells CD80/4-1BBL-T-cells
The effect of compounds on cell vitality was determined by MTT assay in various cells.
The cell vitality after labeling was compared with that of unlabeled cells and expressed as the relative ratio.
In other words, the cell vitality has an extremely close relationship with the geometric factors of nanotube openings.
In order to select an appropriate concentration of H2O2 to build oxidative stress-impaired cell model, incubated cells with different concentration of H2O2 for 12 h then determined cell vitality with MTT method, as shown in Fig. 8a, when H2O2 concentration was 700 μM, the cell vitality was 74.77%, when H2O2 concentration was 800 μM, the cell vitality was 71.51%.
Scanning electron microscopy (SEM) and cell vitality assessment indicated significant osteoblastic proliferation on the surface of the scaffolds.
Cell vitality as assessed by XTT assay was significantly better in the JSD group as compared to static culture.
We conclude that these metabolic traits are essential for sustainment of cell vitality and thus, a more efficient infection response.
Cell vitality staining and biocompatibility tests showed superior biocompatibility of HA scaffolds to BioOss®, while BioOss® was more compatible than TCP.
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