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(Bottom) A cell treated for 90 min with 10 µM U0126 to inhibit Erk.
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Control cells differed from etoposide-treated cells in all conditions except in PHA-stimulated cells treated for 24 h.
Figure 8 Transmission electron micrographs of Hela cells treated for 3 h with FITC-loaded HMSNs.
Furthermore, cells treated for 4 h with one lethal dose of 1 10 phenanthroline and ρ-aminobenzamidine, caused serious damage to the intracellular vacuolar system and nuclear material.
Fluorescence was scarcely detected in nearly all cells treated for more than 10 min. Figure 1 β-Galactosidase activities by the cells treated with chloroform and heat treatment at 20 70°C for 10 min.
In the nanoscale Photosan group, significant differences in cell viability were observed after 1 and 2 h of incubation, whereas cells treated for more than 2 h exhibited no significant differences in cell viability (Figure 1A).
Cells treated for 60, 120 and 360 min or not were harvested and cell lysates were prepared for immunoprecipitation with anti-Smad2/3 followed by immunoblotting with anti-Smad4 mAb or anti-FoxO3 pAb.
We carried out the CTC staining of cells treated for 5, 10, 15, and 30 min at 45°C to determine heat treatment time for the inactivation of basic metabolism in Ac10-Rifr cells.
These genes were not elevated in cells treated for 24 h with 1 µM retinol alone.
Changes were also observed for B6 and B7, but not for cells treated for shorter periods of time.
Cells treated for 10 min with 10 µM PHE present numerous surface invaginations corresponding to caveolae (Figure 3A, b) as compared to non-treated cells (Figure 3A, a).
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