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Influenza viral mRNAs are translated by the host cell translation machinery ('Translation' in Figure 1); thus not surprisingly, several cellular translation factors such as eIF4A (eukaryotic initiation factor-4A), eIF4E, and eIF4G interact with viral mRNAs and/or polymerase complexes [ 95- 98].
Bacteriophage Qβ utilizes some host cell translation factors during replication.
These data are novel observations showing that Stxs regulate multiple signal transduction pathways controlling translation in host cells, and support a role for eIF4E phosphorylation in maintaining host cell translation despite ribosomal intoxication.
Since the cell translation machinery recognizes Inosine as Guanosine, editing in coding sequences can recode an amino acid and therefore affect the protein structure and function [7], [13], [15], [16], [17].
In addition, the unique mechanism of calicivirus VPg-dependent calicivirus translation initiation which relies on the interaction of host cell translation initiation factors with the VPg protein covalently linked to the 5' end of viral RNA [29], [30], [31], precludes the use of in vitro transcribed RNA for the study of authentic virus translation and replication.
Photobleaching and photoactivation data were corrected for cell translation and rotation in the stack_reg plug-in.
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PDCD4, a 64 kDa protein, is an important, recently identified tumor suppressor that inhibits cell transformation, translation and invasion.
Non-natural amino acid mutagenesis has enabled us to incorporate modified amino acids into specific positions of proteins in both cell-free and in-cell translation systems using tRNAs that are aminoacylated with modified amino acids.
In mammalian cells, translation termination codons and exon exon junctions are cis-acting elements that allow recognition of PTCs.
In normal resting cells translation initiation may be very low as a large fraction of eIF4E may be complexed with the inhibitory 4E-BPs in the absence of Akt/mTOR pathway stimulation [28].
(A ) Schematic representation of the live-cell translation reporter.
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