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It can be beneficial to allow the stronger cell to exclude the weaker cell, in a way that will lead to affinity maturation of the T cell response.
Briefly, three lines were drawn across each cell to exclude the nucleus, and the Pearson correlation coefficient (r) was computed for the relationship between the intensities of the two fluorescence channels (green for IP3R fragments and red for calreticulin).
Integrity of the plasma membrane is shown by the ability of a viable cell to exclude the dye, whereas the dye will diffuse passively into sperm cells with damaged plasma membranes [ 22].
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First, further investigations are needed in human primary alveolar epithelial cells, to exclude the possibility that the profile of secreted lipid mediators could differ.
Trapping and identifying EGFR-expressed single cells to exclude interferences from EGFR-unexpressed cells.
Viability was measured by the ability of cells to exclude Trypan blue dye (Invitrogen).
We also (over)expressed only TRX-1B in ASJ cells, to exclude light-independent effects of TRX-1B on dauer-entry or -exit.
We used HeLa cells to exclude the involvement of caveolins in the interactions of host cells with OMVs because these cells did not harbor caveolins in the cytoplasmic membrane [25].
We did not collect any floating cells to exclude dead cells and debris.
Cell death was detected based on the inability of cells to exclude vital dye PI.
Next, we tested for the localization in human tubular cells to exclude cell line- or species-specific localization of ANO6.
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