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I had a feeling that a cell search was coming that week so I had cleaned my cell thoroughly.
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From Amorepacific, the Treatment Enzyme Peel ($50 at Bergdorf Goodman) features papain, an enzyme derived from green papaya described as able to slough off dead skin cells thoroughly but gently.
Briefly, 2 μL of the cloning reaction were added to E. coli cells, thoroughly agitated and incubated in ice for 10 min.
Cells were next disrupted by using Dounce homogenizer (Wheaton, Millville, NJ) for 25 strokes and gently rocked on an orbital shaker at 4°C cold room for 15 min to lyze cells thoroughly.
To compare the dynamics of AJ assembly in nontransformed and transformed epithelial cells thoroughly, we investigated cell-cell collisions in a narrow wound.
We found that it is important to wash NK cells thoroughly after preactivation, that is at least three times with a large volume of PBS.
When developing or implementing novel culture conditions it is important to characterise the cells thoroughly in order to validate the culture system.
To demonstrate that the anti-inflammatory effect of hBD3 was not simply due to hBD3-LPS binding, we treated RAW264.7 cells with hBD3 for 1 h and washed the cells thoroughly before stimulating with LPS.
Even before the discovery of grid cells stellate cells were thoroughly studied because of their rapid membrane time constants and resonant behaviour.
After disposal of non-adherent cells, and thoroughly washing the adherent cells with phosphate-buffered saline (PBS) twice, cells were recovered using 0.5% trypsin (Gibco).
Cell pellet thoroughly mixed with the cell fixative was applied on the formatted and coated CTC slide.
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