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To quantify, images were deconvolved using a 2D fast deconvolution function with settings of thick (cell thickness), noisy (image noise level) and strong (contrast enhancement).
The integument is composed of layers that may be of single cell thickness, as in many invertebrates, or multiple cell thickness, as in some invertebrates and all vertebrates.
Cell thickness was measured by cross-section analysis of the RBC images.
This ratio is independent of the biosensor's fluorescence intensity, so eliminates the effects of cell thickness, uneven biosensor distribution, uneven illumination and other factors.
Check that PML parameters are sensible (e.g. that total PML thickness is no greater than cell thickness) to avoid common mistakes.
The dependence of this optimum on cell thickness and base resistivity was also analyzed.
In these studies we used a laser gauge for monitoring the cell thickness variation under operation.
Apoptosis was confirmed by the quantification of morphological parameters (reduced surface area, increased cell thickness) and positive labeling with annexin V. Autophagy was confirmed by monodansylcadaverine staining, identifying similar autophagic vacuoles with both Cd2+ and CdTe-QD.
The average cell thickness of the keratocytes was 1.34 ± 0.46 μm (range 0.49 4.76 μm), with the apparent cell thickness being related to the average anterior posterior thickness of the adjacent lamellae (r= 0.424, P= 0.001) ).
These studies indicate that the keratocyte cell thickness is influenced by the physical pressure exerted by adjacent stromal lamellae.
A correlation between capacity retention and cell thickness variation is demonstrated, which could be potentially incorporated into Battery Management System BMSS) design for Li-S batteries.
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