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When blood components (e.g., leukocytes and platelets) adhere to a surface (e.g., blood vessel wall), shear flow causes the elongation of the non-adherent part of the cell membrane forming a long thin cylinder shape (i.e., cell tether).
The formation of cell tether is important for regulation of cell adhesion strength and stabilization of cell rolling, and may significantly affect the flow dynamics inside the vessel, as well as the motion of other cells and bioactive molecules.
Although significant efforts have been made to reveal mechanisms underlying cell tether formation, the role of nucleus, nucleus/cell volume ratio, nucleus/plasma viscosity ratio and cytoplasm/plasma viscosity ratio remains unknown.
Similar(57)
Our optical trapping technique is general, since it does not rely on cell tethering, and therefore should be applicable to different bacterial species.
A distinctive advantage of our technique is that it does not rely on cell tethering and thus generally applicable to different bacteria.
Cytokinesis in the insect (procyclic) form of T. brucei is initiated at the anterior end of the daughter cell tethered to the dorsal mid-point of mother cell and proceeds in a helical manner following the new flagellum/flagellum attachment zone (FAZ) as axis [5], [7].
This further indicates the specific role of P-selectin for cell tethering rather than cell rolling.
At shear stresses of 5.0 and 10.0 dyn cm 2, no cell tethering could be observed.
Initial cell tethering and filopodia exploration are followed by lamellipodia ruffling membrane activity and cellular spreading.
Whereas uMUC1 interactions with E-selectin supported cell rolling, P-selectin: uMUC1 interactions exclusively facilitated cell tethering, while L-selectin surfaces supported no cell adhesive interactions.
Moreover, uMUC1 interactions with P-selectin exclusively facilitated cell tethering events on P-selectin-only and E- and P-selectin combined surfaces whereas L-selectin surfaces produced no cell interactions.
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