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The iPS cell technique "is amazingly efficient," he says.
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Jenike's shear cell technique was applied to evaluate the wall friction, in particular, the wall yield locus and angle of wall friction, and to evaluate the minimum hopper angle required for mass flow.
The conventional whole cell technique was used only in those experiments in which cells were dialysed with Cs+ -based solution.
"We think this opens new possibilities in regenerative medicine...One possible advantage over other stem cell techniques is that the cells are already there in the right place with the possibility of becoming functional cells - with no need for grafts," said Manuel Serrano of the Spanish National Cancer Research Centre in Madrid.
A finite-difference marker-in-cell technique is used to solve for the thermo-mechanical problem including complex visco-plastic rheologies in high resolution.
A newly implemented cut-cell technique was employed to deal with the curved surface of submerged tubes.
The standard whole-cell technique was used with the sine+DC lock-in function of an EPC10 amplifier and Patchmaster software (HEKA Electronik, Lambrecht/Pfalz, Germany).
Additionally, the cut-cell techniques are utilized for handling an arbitrary geometry, and an absorbing-generating boundary condition for a wave generator is applied.
More sensitive single-cell techniques are required for a better understanding of persister mechanisms.
Independent of the origin of the barrier forming cells, techniques are needed to quantify their barrier integrity.
The single-cell techniques are powerful for demonstrating tolerance to cidal antibiotics in a single persister, yet so far no transcriptomic or proteomic data are available for single persister cells due to the lack of sensitivity of the current methods.
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