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It is the latter issue where the metallomic approaches have received growing attention as indispensable to decipher the metabolic pathways, to shed light on modes of delivery and action, and to identify active, toxic species and potential cell targets of metallodrugs.
Most studies of pathogens exhibiting placental tropism, such as human cytomegalovirus [14], Toxoplasma gondii [15] or Brucella abortus [16], have used placental tissues but were unable to define the cell targets of the infection.
In addition, although KC cells have so far shown to be a good in vitro representation for C. sonorensis (from which larvae these cells have been generated) [23], [40], BHK cells, a standard cell line commonly used for BTV in vitro studies, are not suited to represent all of the so far identified numerous mammalian cell targets of BTV.
In order to address the importance of the 5103 fragment AP-1 binding sites for HIV-1 replication in the two major cell targets of HIV-1, i.e. T cells and macrophages, we infected T-lymphoid Jurkat and promonocytic U937 cells with the wild-type and mutant viral stocks.
In order to determine immune cell targets of VV within the CNS, we infected wild type mice with recombinant VV expressing enhanced green fluorescent protein (VV eGFP).
We first investigated in vitro leptin and amylin signaling in human primary adipocytes (hPAs) and human peripheral blood mononuclear cells (hPBMCs), known cell targets of leptin action.
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Amphiphilic cyclodextrins obtained by appending a hydrophobic anchor were designed to improve the cell targeting of the drug-containing cavities through their liposome transportation in the organism.
The expression of TC in ER did not modify the cell targeting of multiligand proteins that share affinity for TC.
Thus, using the CPE peptide, M cell targeting of mucosal vaccines might be possible in humans.
For example, Badruddoza et al. in 2013 synthesized uniform nanocomposite for cell targeting of hydrophobic compounds.
These experiments demonstrated that OTBCs had an epithelial origin, and the cell target of OCT4 was possibly a primitive stem/progenitor cell.
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