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These two examples demonstrate how important is to consider that ExMVs released during cell purification may change cell surface phenotype.
By cell surface phenotype and vasculogenic potential in vitro and in vivo, we also found the ECFCs to be extremely similar to AT-ECs.
In our experience the sensitivity of FACS allows for the detection of 5 fetal in 107 maternal cells and assessment of cell surface phenotype.
As few as 100 cells bearing this phenotype were capable of forming tumors in immune deficient NOD/SCID mice, whereas 10,000 cells without this cell surface phenotype were non-tumorigenic.
Peripheral blood mononuclear cells from healthy subjects were cultured in the presence or absence of different combinations of IL-17s and changes in relative numbers and cell surface phenotype of NK cells and CD56+CD3+ cells measured by flow cytometry.
Molecular imaging using engineered antibodies and fragments provides a general approach for assessing cell surface phenotype in vivo and stands to play an increasingly important role in cancer diagnosis, treatment selection, and monitoring of molecularly targeted therapeutics.
Firstly, what cell surface phenotype correlates with HSC activity before and after in vitro expansion?
These cells exhibit a normal cell surface phenotype and some functional activity.
Next we assessed the cell surface phenotype before and after culture.
The number and cell surface phenotype of liver NKT cells recovered by perfusion and by the traditional method were comparable.
Thus, the DN4 population is different from the αGC/CD1d dimer+ NKT cells in terms of cell surface phenotype.
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