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Also, the pre-formed effector protein pools in the bacterial cytosol were somewhat elevated in the HilA-over-expressing strains (∼25000 molecules SipA, ∼25000 molecules SopE and ∼25000 molecules SptP per bacterial cell; Supplementary Fig. S1B and data not shown).
The inter-cellular model we use is two dimensional (2d), and the medium is represented by a square lattice, where each lattice square has the size of a single cell (Supplementary Figure S7).
In contrast, very few (4%) of SNX1 −/− BMDM depleted of SNX5 had >10 macropinosomes per cell (supplementary material Fig. S2).
In these cells Prestin YFP shows decreased targeting to the basolateral surface of the cell (supplementary material Fig. S2).
Promoted p62 Keap1 interaction was also observed in HepG2.2.15 cells compared with HepG2 cell (Supplementary Figure 3a).
The mitochondria did not migrate at uniform speeds and met together before entering the UV-treated cell (Supplementary movie 3).
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Similar experiments were performed using HEK293 cells (supplementary Figure S4).
These lysosomal effects did not however affect neuronal survival in these cycling cells (Supplementary Figure S1B).
We found that apoptotic cells with fragmented nuclei lacked actin microfilaments and AMN presented the typical 'cocoon' like structure that may function as a protective barrier surrounding the whole-cell (Supplementary Figure S1).
Comparable results were observed in PC3 cells (Supplementary Fig 9B).
Similar results were obtained with SW480 cells (Supplementary Figure S2).
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