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Interestingly, Ybp2 localizes throughout the cell (Supplemental Figure S7, [36]).
Indeed, AATF was mainly localized to the nucleus and the nucleolus in the cell (Supplemental Figure 4).
Non-irradiated cell lines are characterized by low levels of chromatid fragments, with average frequencies detected ranging from 0.01-0.08 per cell (Supplemental Table S1).
Before nuclear envelope breakdown (NEB), MCAK localized to all MT plus-ends, independently of their position within the cell (Supplemental Fig. S 2b; Fig. 2b, c), but as soon as NEB was observed (as determined by chromosomes that had moved out of the nucleus), MCAK association with MT tips in the vicinity of chromosomes was strongly reduced (Supplemental Fig. S 2c; Fig. 2b, c).
Similar(55)
Similar results were obtained in PANC-1 cells (Supplemental Fig. S1).
Similarly, CD69 was up-regulated in splenic NK cells (Supplemental Fig. S1).
Expression of p53 was not observed in surface epithelial cells (Supplemental Figure S3).
Similar results were obtained in siTcl-transfected ES cells (Supplemental Figure S4B).
Similar results are also obtained by analyzing the Ntv-a/pCt and Ntv-a/pUP cells (Supplemental data S12).
An additional nine miRNAs that were more highly expressed in S cells (Supplemental Fig. S2) will be investigated separately.
On the other hand, ssDsbA-frGFP is efficiently degraded and does not accumulate anywhere in cells (Supplemental Fig. S1e).
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