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We describe the design of micropatterned surfaces for single cell studies, based on photo-patterned thermoresponsive polymer brushes.
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Cell therapy studies based on mesenchymal stem cell (MSC) transplantation noted the potential benefit of neural induction by neurogenin 1 from transplanted MSCs in the delay of disease onset and in the enhancement of motor functions in transgenic SOD1G93A mice [ 82].
Moreover, genetic and cell biological studies based on the differential expression of mutant SOD1 in distinct cell types have demonstrated that although death of MNs causes ALS symptoms, the disease also renders other cells, such as astrocytes and microglia, dysfunctional.
Large-scale functional RNAi screens will be possible, together with more conventional small molecule screening, in the hope that future cell culture studies, based on more physiologically representative models, will be more readily translated into clinically relevant findings.
The observation that the function of SLX4 in ICL repair depends on the FA ubiquitination pathway in chicken DT40 cells awaits confirmation from studies based on human cellular systems.
Here, we validate the kinases with high KAR rankings (Table 1) in a subset of the TNBC cell lines studied based on previously published studies.
In conclusion, this comparative C. elegans-human cell lines study based on orthology assignments explores gene functionality by combining two key aspects of functional genomics: a whole organism approach and protein overexpression data using cells lines.
This study demonstrates the resolution of intratumor heterogeneity for profiling studies based on cell proliferation, a defining feature of cancers, with implications for treatment design.
Studies based on cell cultures support this evidence.
SPECT imaging allows whole body biodistribution studies based on cell labeling with In-oxine.
Authors' response: Based on our experience we suggest using at least 3 replicates for studies based on cell lines.
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