Sentence examples for cell spots from inspiring English sources

The antibody-secreting cell spots were recorded per 106 input cell numbers.

After in vitro stimulation of cells with the M2 specific peptide, cytokine producing cell spots were measured as an indicator of T cell responses (Fig. 5).

Following rehydration in PBS, cell spots were incubated with ∼1 µg purified NKp46-Ig, NKp44-Ig or NKp30-Ig in 50 µl PBS/2% FCS for 45 min at room temperature.

Interferon (IFN)- γ and interleukin (IL -4 secretIL -4ell secretinge determined on Multi-scelln 96 well platespotsllipore, Billerica, MA) coated were cytokine specific capture antibodeterminedscribed [53].

To avoid false positive results in the ASMA staining on the freshly isolated CD68+ cell spots and as the mouse monoclonal CD68 antibody was already attached to the cells, these were blocked with goat anti-mouse/biotin (GAM/B) for 5 h prior to the actual staining procedure.

As for the immunofluorescence test, undiluted samples were layered on HEp-2 cell spots and incubated for 30 minutes.

We're going through a bad cell spot here and I can't hear your (unintelligible).

Before cell spotting the slide was rinsed in ultra pure water before surface cleansing with a 20% nitric acid bath under agitation for 20 minutes.

JKR and OP performed RNAi-based cell spot microarray screenings.

Objective and rapid analyses of cytokine producing cell numbers (spots), and relative amounts of cytokine produced per cell (spot size) are now possible.

We describe here the optimization and functional application of a miniaturized cell spot microarray (CSMA) method for RNAi screening.