Exact(13)
Equating the experimental and simulated mean cell speeds
For final validation of cell migration, individual cell speeds were measured using time-lapse videomicroscopy.
A Microsoft Excel macro was used to calculate average cell speeds, as described below.
Within seconds, SACY produces cAMP and, thereby, causes the flagella to beat faster so that the sperm cell speeds toward the egg.
Higher laser powers of 106 mW/μm provide F optical of 9.98 pN and theoretical cell speeds of ~160 µm/sec.
Migration speed (μm/minute) was calculated as the average of cell speeds (migration distance between the current frame and the previous frame divided by the time between sequential frames, 0.5 minutes) at each captured frame.
Similar(47)
It is concluded that LDF signal derives predominantly from nonvectorial cell speed and less from cell number.
This paper presents a very simple dynamical mathematical model directed toward elucidating the dependence of cell speed on receptor and ligand number densities and receptor ligand binding constants.
This compares with the cell motility rate on a flat PMMA surface where the average cell speed is around 0.012 μm/s.
Further, there was a monotonic increase in cell speed and spreading with increasing adhesivity that showed a lack of the biphasic trend seen in 2-D cell migration.
Addition of 100 nM S1P increased cell speed and random motility coefficients on both RGD peptides, with the largest increases found on cyclic RGD.
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