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HDF was treated with 20 μg/mL GO and cultured in a humidified 5% CO2 balanced air incubator at 37°C for 24 h, then fixed cells with 2.5% glutaraldehyde solution and embedded with epoxy resin, finally made the ultrathin cell specimen and observed the specimen with TEM.
The pixel resolution obtained with this lens is 254 nm per pixel on the cell specimen.
The pixel resolution was 166 nm/pixel on the cell specimen.
Among them, a cell specimen, a part of which had been cryopreserved as living cells, was included.
The cell specimen was analyzed on the FACScan and antibody-binding capacity calculated by interpolation on the calibration curve.
A cervical cell specimen was collected from each control using a cytobrush, and tumor biopsies were obtained from all cases.
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The authors are grateful to Anaid Lugo Leija, Ksenija Velickovic and Virginie Sottile (Wolfson STEM Centre, U. of Nottingham) who provided adipose cell specimens.
After validating with Sanger, the current "gold standard" of sequencing, in 89 specimens with concordance of 99.9%, we used our NGS method and custom annotation pipeline to sequence 796 HPV16-positive exfoliated cervical cell specimens.
A national repository of tissue and cell specimens is also part of these NIH-supported trials to further promote scientific study from these unique patients.
Cell specimens were dehydrated and embedded in Epon (Epon-812; Fulham, Latham, NY).
(ii) Immuno-EM analysis of cell sections Cell specimens were included in metacrylate resin (LR White Resin, London Resin Company, Reading, UK).
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