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Flow cytometry and fluorescence activated cell sorting techniques were designed to realize configurable classification and separation of target cells.
Using yeast cell surface display and fluorescence activated cell sorting techniques, we have engineered an NY-ESO-1 variant (NY-ESO-L5; C75A C76A C78A L153H) with a 100× improved display level on yeast compared to the wild-type protein.
More recently, cell sorting techniques reliant on fluorescently tagged transgenic marker proteins have been employed to purify specific cell types and thereby refine the screen for potential regulators of gonadogenesis [21], [22].
Fluorescence-activated cell sorting techniques were used to recover cells selectively from different depths (different oxygenation status) within the spheroids.
Thus, a 48 hr "washout" period or cell sorting techniques should be considered to prevent this type of sampling bias.
Lymphocyte phenotypic subpopulations can be readily isolated from the joint fluid by fluorescence activated cell sorting techniques.
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Unfortunately, T-cell numbers in blood of TB-infected mice could not be determined, since the fluorescence-activated cell sorting technique using Mtb-infected blood samples could not be performed at our institute due to safety regulations.
(2010) used cell-sorting techniques to separate E. coli K-12 from Pseudomonas putida KT2440 in a mixed culture of 5 × 10 cells and applied proteomics to identify proteins that were expressed in each subpopulation.
By fluorescent-activated cell sorting technique, it was found that the cytotoxicity of the nanoparticles is cell-specific.
After several passages until overgrowing of fibroblasts, positive selection of fibroblasts was isolated by magnetic cell sorting technique (MACS separation, Miltenyi, Bergisch-Gladbach, Germany) as previously described [44].
For CD45+ and CD45- renal cells isolation kidneys were minced, passed through 70 and 30 µm cell strainers, and positive selection of CD45+ cells was performed by magnetic cell sorting technique (MACS separation, Miltenyi, Bergisch-Gladbach, Germany) as previously described [44].
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