Sentence examples for cell sorted from inspiring English sources

For each cell population, two to four biological replicates (different mice) were prepared, and two to three separately sorted cell samples from each mouse were cell sorted and analyzed.

Only cell sorted GFP positive cells were used in animal experiments.

We therefore cell sorted several different CD19+CD27+ B cell subsets from individual HIS mice.

Of note, immunohistochemical analysis of cell sorted CD4+ subpopulations revealed that anti-FOXP3 immunohistochemistry as used here did only detect CD4+CD25+ cells with high FOXP3 expression (data not shown).

TN-XXL gene expression was introduced into each of the α7-Ric3 and α4β2 cells via lentiviral transduction as described [21] and fluorescence activated cell sorted (FACS) for high levels of eCFP and Citrine cp174 fluorescence.

Pentamer positive cells were single cell sorted into 96-well round bottom plates containing 1×105 irradiated autologous feeder cells and 5×103 LCL using a FACSAria multi-color high-speed sorter (BD Biosciences).

Single-cell sorted CD4+CD25neg or CD4+CD25+ cells obtained from 6 NC were incubated±RAPA, stimulated with the relevant cytokines (150 IU/mL) for 20 min, permeabilized and treated with phosphorylated (phospho) STAT5 or STAT3 Abs.

In fact, all the possible amplification techniques could be applied on the single-cell sorted CellSearch CTC.

Analysis of 16S rRNA gene libraries constructed from Fluorescence Activated Cell Sorted-recovered fluorescent populations (n = 3), revealed that 98.5% (Pseudomonas spp. comprised 68.7% and Burkholderia spp. 29.8%) of the total sorted population was specifically targeted as evidenced by the homology of the 16S rRNA sequences to the probe sequence.

Furthermore, the UHRF1-GFP wt, H346G and H730A cell lines were single-cell sorted.

DsRed-expressing cells were single-cell sorted on an Influx instrument (Cytopeia) and cultured in 2 mg/ml G418 (Invitrogen) for isolation and maintenance of stably integrated clonal events.