Sentence examples for cell sieve from inspiring English sources

The digested tissue was centrifuged at 100 rcf for 5 minutes, after which the pellet was resuspended in 1 ml of neurosphere growth medium, mechanically triturated, then filtered through a 40 µm cell sieve (Falcon/BD Biosciences, Sydney, Australia).

The tumor cells were isolated from excised skin tumors by passaging through a cell sieve to remove connective tissue and MACS with antibodies for the Vβ family of the tumor cells as previously established by immunohistochemistry.

The tumor cells were isolated from melanoma metastases by treating minced tissue with collagenase free of contaminating protease activities and DNAse, passaging the resulting suspension through a cell sieve to remove connective tissue, allowing the tumor cells to adhere to tissue culture plastic plates and wash off non-adherent cells.

It was then passed through a 70 μm cell sieve, crushed with a syringe plunger and rinsed with the medium.

The resulting suspension was filtered through a 70  μm sterile cell sieve (BD Biosciences, MA, USA) and centrifuged at 720 g for 5 minutes.

Primary cortical neurons were prepared from the brains of mouse embryos (day 15.5) after mechanical dissociation, cell sieving and isolation on histopaque (Sigma).

Primary cortical neurons were prepared from the brains of mouse embryos (day 15.5) after mechanical dissociation, cell sieving and isolation on histopaque (Sigma, Poole, UK).

Cells isolated from porcine NP tissues were separated with three cell sieves, i.e., cells > 20 μm, 8-20 μm and cells < 8 μm (CellMicroSieves, BioDesign Inc., New York, USA) [ 33] with the goal to identify NPC and NC.

Additionally, notochordal cells were separated from NP cells using enzymatic digestion as described above and the cells in the supernatant separated further by using a sequential cell sieving method [ 16].

Nutrient-conducting cells—sieve cells and sieve tube members (which in wood correspond to the axial tracheids of softwoods and the vessel members of hardwoods, respectively)—are thin-walled and nonlignified, serving usually only one season of growth.

The highly specialized conducting cells, sieve elements (SEs) are connected by perforated sieve plates to form sieve tubes; a living, functional conduit of cells that allows low resistance movement of sap throughout the plant (Oparka and Simon 2000).