This approach includes cell sheet stacking [1],[2], cell sheet sandwiching [3], cell sheet wrapping [4],[5], and 3D cell accumulation [6],[7].
For obtaining a stable stacked cell sheet construct, a sophisticated 3-D cell sheet manipulation system using temperature-responsive culture dishes and a cell sheet manipulator was developed.
One is an allogeneic corneal epithelial stem cell sheet, and the other is an autologous oral mucosal epithelial cell sheet.
One of the techniques is cell sheet engineering, which requires creating a contiguous cell sheet and harvesting it safely.
Three different cellular constructs, including micropatterned cell sheet, round flat cell sheet, and spherical aggregate, were fabricated by our device to demonstrate the applicability in biological applications.
In tenogenesis, the so called cell sheet technology has been preferred (Markway et al. 2013).
A cartoon model of cell sheet formation is depicted in Fig. 3.
Cell sheet thickness and cell metabolic activity increased in the second and third week.
This cell sheet has a pattern of microhole array, and thickness of about 100 μ m.
After 100% confluence, cell sheet was detached by rinsing with saline.
Fig. 3 Cartoon of the procedure to form tendon-like cell sheet.
