For further enrichment, a negative selection for CD11b positive cells was performed using a MidiMACS magnetic cell separator, LD separation columns and CD11b magnetic microbeads (all from Miltenyi Biotec, Bergisch Gladbach, Germany).
Cell separation was performed on an AutoMACS Cell Separator, as recommended by the manufacturer.
After 20-minute incubations on ice, and at 37 degrees C, the pancreas was hand shaken for 1 minute, followed by filtration and separation on an automatic cell separator (COBE 2991).
Cells were washed, incubated with anti-FITC MACS beads for 15 min at 4°C, and then separated on an autoMACS cell separator, according to manufacturer's directions.
A cell separator design is presented for rapid, efficient separation of analytical quality plasma samples from whole blood for use in a continuous segmented flow point-of-care testing system.
5T33MMvv cells at a concentration of 107 cells per 90 µl MACS buffer (phosphate buffered saline containing 0.5% bovine serum albumin and 2 nM EDTA, pH 7.2) and 10 µl CD11b Microbeads were incubated at 4°C for 15 min. The 5T33MMvv-microbeads mixture was then loaded onto a separation column placed on the magnetic cell separator.
Synovial macrophages were isolated by positive magnetic separation with CD14-conjugated magnetic beads (MACS; Miltenyi Biotech) and a magnetic cell separator (MACS) in accordance with the manufacturer's protocol.
This new cell separator is an improved version of the Fresenius AS-TEC 204 cell separator, designed to allow more efficient platelet collections.
After incubation in UW solution for 30 min, the digested tissue was purified using UIC-UB gradient42 in a Cobe 2991 cell separator (Cobe 2991, Cobe, USA).
The efficacy of the cell separator (52.44±7.35%) is comparable to that of other separators.
Here, we describe a new immunomagnetic cell separator, the MagSweeper, which gently enriches target cells and eliminates cells that are not bound to magnetic particles.
