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(A ) Lateral view of a single animal showing prominent expression in an amphid sheath cell, seam cells, and the syncytial hypodermal cell (hyp7) in a J4 hermaphrodite.
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Embryonic elongation occurs as a result of contractions of the actin cytoskeleton of the lateral epidermal cells (seam cells) (Priess and Hirsh 1986; Chisholm and Hardin 2005; Gally et al. 2009).
Here, we demonstrate that intracellular phospholipase A1 (PLa1), a poorly characterized lipid-metabolizing enzyme, controls the subcellular localizations of β-catenin in the terminal asymmetric divisions of epithelial stem cells (seam cells).
The sensory rays are involved in locating the hermaphrodite vulva during copulation and are secreted by the lateral hypodermal cells, the seam cells.
These so-called heterochronic phenotypes are particularly apparent in a subset of skin cells, the seam cells.
ced-3 may act upstream of multiple conserved pluripotent factors to affect differentiation of stem cell-like seam cells.
Cell-specific RNAi experiments revealed that the retrograde trafficking acts cell-autonomously in seam cells to modulate the asymmetric cortical localization of WRM-1.
In fixed, permeabilized worms, BAH-1 was detected in association with hypodermal cells, including the seam cells, consistent with the promoter fusion data (Figure 4B).
As mentioned above, ipla-1 functioned cell-autonomously in seam cells (Table I, see group A).
Taken together, all these results indicate that mon-2 and tbc-3 function cell-autonomously in seam cells for their interactions with ipla-1.
These results indicate that ipla-1 functions cell-autonomously in seam cells to regulate their S4 divisions through its enzymatic activity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com