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The multi-wavelength cell scoring application module of the analysis software MetaXpress was used to quantify, at the single cell level, the intensity of cPARP staining (200 was used as the intensity above background for cPARP images).
The multiwavelength cell scoring module of the Metamorph software allows for image analysis.
First, different cell scoring methods resulted in bias regarding the assignment of high and low lymphocyte infiltration.
Images were processed and quantified using the multiwavelength cell scoring application module in MetaXpress software (Molecular Devices).
A multi-wavelength cell scoring algorithm within the MetaXpress software (MetaXpress 2.0, Molecular Devices) was used to obtain total image intensities.
Potential inter-pathologist variation in tumour cell scoring (+/- 10%, based on Figure 1) was taken into account in the learning model by randomly adjusting the training TCP during each iteration with -10, 0 or 10percentt.
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An estimated 700-fold enrichment for homologous versus nonhomologous integration events was obtained, such that 70% of cells scoring positive for Thy-1 were derived from gene targeting.
The intensity of the cells scoring moderate or higher was judged as positive.
After amputation, the fish were incubated in the JNK inhibitor (SP600125) or vehicle and TUNEL-positive cells scored at 18 hpa (Fig. 5b).
The signal pattern was consistent in 100% of interphase and metaphase cells scored.
The analysis of aqueous cells score, flare scores, cell counts, and flare value was conducted with a repeated measures analysis of variance at postoperative visits.
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