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Analysis of cellular DNA damage was carried out by fluorescence microscopy, using a fully automated cell scanning system Metafer-4 (Metasystems, Altlußheim, Germany).
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To observe Chr21 proteins within each cell cluster, a BioCCD scanning system (Applied Biosystems, Darmstadt, Germany) was used.
HCT-8, and MCF-7 cells and HEK293 transfectants were analyzed with a fluorescence laser scanning system (TCS-SP2 scanning system and DM IRB inverted microscope, Leica, Solms, Germany).
The cells were then examined by a confocal laser scanning microscope, Laser Scanning System LSM 510 (Carl Zeiss, Jena, Germany).
Cells were examined at 100× magnification with a Leica TCS SPII confocal laser scanning system equipped with a water-cooled argon-krypton laser.
The cell lysates were then incubated with the secondary antibody and visualized with an Odyssey infrared scanning system.
Cells were visualized using a Zeiss Axioplan 2 microscope with a 510 Meta confocal laser scanning system.
The presence of Ki67-positive tumor cells and CD31-positive vessels in rat stroma tissue was analyzed using a fluorescence laser scanning system (TCS-SP2 scanning system and DM IRB inverted microscope, Leica®, Solms, Germany).
Cells were analysed using a Leica DMI6000 microscope equipped with a Leica TCS-SP5 multispectral confocal laser scanning system.
The cells were viewed with a LEICA DM4000B fluorescence microscope equipped with a Bio-Rad MRC1024 laser confocal scanning system and a LEICA DC500 camera.
IHC-stained slides were scanned and the total positive cell numbers and intensity of anti-Collagen IV or anti-HUIV26 staining was computed and measured by ImageScope from Aperio Scanning System (Leica Microsystems Inc., Buffalo Grove, IL).
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