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Cell samples were stored at −20 °C until analysis.
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The cell lysates samples were stored at −80 °C for further use.
The cell extract samples were stored at −80°C until drug analysis by HPLC.
The culture supernatants were collected and cell-free samples were stored at -30°C until the cytokine assay.
Culture supernatants were collected 36 hours later and cell-free samples were stored at -30°C until cytokine assay.
All the cell-free plasma samples were stored in aliquots at −80 °C until assayed collectively by an investigator who was blinded to patient assignment.
Samples were centrifuged at 1000g for 15 min to remove cells and serum samples were stored at −80 °C until analysis.
Cell-free SF samples were stored at -80°C.
Except for standard biochemistry and blood-cell analyses, serum samples were stored at -80°C and analyzed at the end of the study.
Cell extracts and RNA samples were stored frozen at -20 °C until further use.
Both cell extract and media samples were stored at −80°C until analysis for mycobacterial infection.
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