Exact(10)
The cell samples were prepared as previously described [15].
The bacterial cell samples were prepared from four liter cultures in the presence or the absence of subinhibitory concentration of rhodomyrtone (0.174 µg/ml) after incubation for 18 h.
Whole cell samples were prepared aerobically.
Cell samples were prepared in radio immunoprecipitation lysis buffer containing protease inhibitors.
iNKT cell samples were prepared in duplicate and three separate donors were used to provide NK cells.
Cell samples were prepared in 5% metaphosphoric acid with or without 1-methyl-2-vinyl-pyridium trifluoromethane sulfonate, a GSH-specific scavenger.
Similar(50)
Empty-cell samples were prepared by diluting electrocompetent cells 5 10× in PBS. 5 μl of cells was applied to pads containing 1 % agarose (Bio-Rad Certified Molecular Biology Agarose) and 1× minimalmediumdiumedium
Lipidomic Analysis of siSMPD4-, siGand-, and siDGAT2-Treated Cells Samples were prepared and LC-MS runs were performed as described above except a shorter gradient was used for all runs (the gradient started after 5 min at 0% B and then increased to 100% B over the course of 60 min).
First, whole cell protein samples were prepared by lysing cells in a buffer composed of 150 mM NaCl, 50 mMTris (pH 8.0), 5 mM EDTA, 1% (vol/vol) Nonidet P40, 1 mM phenylmethylsulfonyl fluoride (PMSF), 20 ug/mL aprotinin, and 25 ug/mL leupeptin for 30 min at 4°C.
Cell lysate samples were prepared using a proportion of the same cells used for cDNA preparation.
Briefly, cell lysate samples were prepared from a population of cells that were counted and assayed for viability.
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