Exact(2)
Cell samples were frozen every 2 weeks for later use.
Cell samples were frozen periodically to allow for assessment of time-course changes after confirmation of transformation.
Similar(58)
All cell and serum samples were frozen and stored at −180 and −80°C, respectively, until analysis.
After 16 hours cell culture supernatants were collected, cell debris was removed by centrifugation, and samples were frozen at -80°C until assayed.
Supernatants were removed from the wells, cell debris removed by centrifugation, and samples were frozen at –80°C.
In all cases, samples were frozen, thawed three times and titrated on BSC-1 cells in duplicates.
Once filtered, cells were killed with a 5 ml rinse of cold 10% TCA before the filtered samples were frozen in liquid nitrogen and stored at −80 °C.
Plasma samples were frozen at −70°C.
Samples were frozen immediately until use.
Samples were frozen at -80°C.
The water samples were frozen.
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