Exact(3)
Cell samples were diluted to approximately 5×105 cells per ml with M9 minimal media and delivered at the flow rate of 50 to 150 cells/sec.
Cell samples were diluted for fifty times by normal saline.
Finally, cell samples were diluted with 400 μl 1× binding buffer and analyzed in a flow cytometer (FACSCalibur, Becton Dickinson, Franklin Lakes, NJ, USA) within 1 hour.
Similar(57)
Packed-cell samples were diluted 3-fold with ddH2O and analyzed in a 10 mm path length quartz UV vis cuvette (Precision cells).
Jurkat cell sample was diluted in phosphate buffered saline (PBS) to a concentration of approximately 5.7 × 105 cells/ml.
Prior to analysis, cell lysate samples were diluted to 50 μg/mL total protein content as determined by a bicinchoninic acid (BCA) assay to ensure a constant sample input across multiple samples.
Depending on the expected number of target cells, the samples were diluted 40- to 1600-fold, and 10 µl of a diluted bacterial suspension was applied to gelatin-coated slides, air-dried and fixed in 100% ethanol.
Serologic screening for HHV-8 infection was performed by indirect immunofluorescence assay by using lytically induced cells; serum samples were diluted 1 40 (8 ).
After white blood cell count, blood samples were diluted in RPMI-1640 medium (Gibco, France) supplemented with 2 mM L-glutamate, 8 µg/ml Gentaline, and 1 mM Sodium Pyruvate in order to obtain 2×106 mononuclear cells/ml.
For the analysis of cell-associated calcium, samples were diluted in 5% HNO3 and calcium concentration was measured by inductively-coupled plasma optical emission spectrometry (ICP-OES, Jobin Yvon JY2000-2) at 393.366 nm.
For each virus, the cells were washed twice with PBS freeze-thawed several times to disrupt the cell membrane then the samples were diluted over a concentration range of 1.5-300 μg ml-1 of polyhedra then assayed against second instar larvae of cotton leaf worm (CLW) as described by Ibara and Federici [ 26].
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