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The sequences found in the baseline PBMCs of the nine patients for whom cell samples were available appeared to be intermixed with baseline plasma sequences.
The study population included all the individuals identified at this clinical site who met the inclusion criteria and for whom frozen cell samples were available using a database that is maintained in our centre where clinical test results are collected at each clinic visit.
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Peripheral blood mononuclear cell DNA samples were available from a previous study of 63 NHP hunters who had a wide range of WB seroreactivity to HTLV (7 ).
Furthermore, among individuals for whom paired and contemporaneous serum IL-6 and PB CD4 T-cell RNA samples were available, clear correlations between IL-6 and the normalised expression of STAT3-inducible genes were seen (figures 4A D; also online supplementary figures S5A D); for example, serum IL-6 measurements correlated with normalised SOCS3 expression: Pearson's R=0.57, p<0.001 (figure 4A).
The analysis was restricted to those tissues for which cell lines, primary tumors and normal samples were available: brain, lung, colon, ovary and kidney.
First, we analysed M. leprae-induced cytokine production by blood cells of RR patients for whom valid samples were available at three time points (Bangladesh: n = 3; Brazil: n = 4; Nepal: n = 3).
Samples were available from n = 117/124 (94%) patients.
Samples were available from 85 eligible patients.
Unfortunately, no longitudinal infant samples were available.
22 samples were available.
Fifty-nine control samples were available.
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