Sentence examples for cell samples using the from inspiring English sources

Total cellular RNA was isolated from the cell samples using the following method: 0.5 ml 2 M sodium acetate (pH 4), and 2.0 ml of 100% ethanol were added to the GITC-lysed blood and the sample mixed and allowed to stand in an ice bucket for 10 min before being centrifuged for 15 min at 15,000 (g) at 4°C.

Cellular DNA was purified from these cell samples using the QIAamp Tissue kit from Qiagen.

Genomic DNA was extracted from cell samples using the Qiagen DNeasy Blood Extraction Kit (Qiagen, Gaithersburg, MD, USA).

totRNA was extracted from the 18 cell samples using the RNeasy mini kit (Qiagen) according to the manufacturer's instructions.

Genomic DNA was extracted from cervical cell samples using the commercial purification system QIAmp DNA Mini Kit (Qiagen) according to the manufacturer's instructions.

RNA was isolated from whole blood or peripheral blood mononuclear cell samples using the Roche MagNA Pure instrument and RNA High Performance kit (Roche, Indianapolis, IN), following manufacturer's protocols (Supporting Information, File S1).

Total RNA was extracted from the infected cell samples using RNeasy RNA isolation Kit (Qiagen, Germany) and reverse transcribed using Superscript II Reverse Transcriptase and Oligo dT primer (Invitrogene, USA), according to the manufacturer's protocol.

Studies were performed to ascertain the inhibition of hypoxia-induced overexpression of HIF-1α protein in the treated cell samples using western blot analysis.

The modulation of expression of a number of identified modulated genes by microarray analysis was investigated using RT-qPCR in the cell samples used for the microarray analysis (for validation of microarray gene expression data), as well as in the cell samples from four other independent experiments.

Conceptually, an ideal gene selected as an internal reference control should have a constant level of expression across the tissue or cell samples used throughout the experiment, and should not exhibit altered expression with diseased or, control tissues, or indeed experimental conditions [ 10].

Primer sequences for collagen type I, collagen type III, biglycan and RPL-32 can be found in Supplemental Table 2. LOX activity was fluorometrically measured in total protein lysates from tissue and cell culture samples using the LOX activity assay (AAT Bioquest) according to the manufacturer's instructions.