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Our results are similar to those reported by mammalian cell reporter gene assays, confirming the utility of our assay in identifying TR subtype-selective therapeutics.
Enhanced Green Fluorescent Protein (EGFP) is the most commonly used live cell reporter despite a number of conflicting reports that it can affect cell physiology.
Similar results were obtained with sFRP2 in HEK293, C57MG and L cell reporter assays.
In view of these findings, the use of GFP as a cell reporter should be carefully monitored.
The extensive use of EGFP as a live cell reporter is based on the presumption that it does not affect (important) cellular functions.
We use an emerald GFP marker cassette to create a human BAC reporter and ES cell reporter line for the early cardiac marker NKX2-5.
Similar(23)
dsDNA stimulation of NFκB endothelial cell reporters resulted in elevated levels of GFP in comparison to Lipofectamine stimulated cells, suggesting NFκB activation by dsDNA (Figure 2a).
Characterisation of the RPELMAL G-actin inteRPELMAL G-actinrescence aninteractiond cell reporter-bysed assays validates the signifluorescencectin-binding residues for proper Manisotropyandon and regulation in vivo.
Modifications: We have rewritten the text to clarify how relative levels of β and δ cell reporters were applied.
Serious attempts have been made to measure and interpret single-cell reporter gene expression and to understand variability in reporter expression among individuals in a population.
This makes sense, as bacterial life essentially thrives in microheterogenic environments and single-cell reporter information can help us to understand the microphysiology of bacterial cells and its importance for macroscale processes like pollutant biodegradation, beneficial bacteria eukaryote interactions, and infection.
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