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The resulting cell remnants are processed for reuse.
Cell remnants were pelleted by centrifugation for 20 min at 13,000 × g at 4 °C.
Physical methods and chemical and biologic agents are used in combination to lyse cells, followed by rinsing to remove cell remnants.
Although several natural and engineered skin substitutes are commercially available, many contain tissue cells or cell remnants, which make quality control difficult, shelf-life under ambient conditions short, or user interface less than friendly.
The microsphere (c) (green star) (H&E × 10 magnification) is entrapped in a small epicardial branch of the left circumflex (LCX) coronary artery and the remaining lumen of the vessel is filled with thrombi and blood cell remnants (c) (green circle).
After removal of unlysed cell remnants and nuclei by centrifugation in the Eppendorf micro-centrifuge (5 min, 10,000 rpm, 10,200×g); protein concentration was determined by standard method, as described [27]; and 10 μg of equal amounts of protein was loaded into 15%% polyacrylamide gel.
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The CD3+ T cell contamination was consistently lower than 1% (while using LD column alone resulted in 2 3% of CD3+ T cell remnant).
For example, miRNA content in both plasma and serum can be influenced by cell remnant contamination from erythrocytes, leukocytes, or platelets.
This indicates an NHS-dependent DNA reduction in the late apoptotic cell that is mediated by an enzymatic process rather than simply by a DNA release from the apoptotic cell remnant.
The liberated filaggrin is an essential component of the SC that participates in the aggregation of keratin filaments into bundles and promote the flattened shape of dead-cell remnants [6], [16], [26], while the amino-terminal domain of filaggrin enters the nucleus, where it may have a role in regulating terminal differentiation [27].
First, as notochordal disc cells are known to express CK8 [ 19], are the CK8-positive cells remnants of the original notochordal population of the disc?
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