Exact(31)
Data analysis for in situ PLA images was performed using the freeware Cell Profiler, developed by the Broad Institute ( http://www.cellprofiler.org ).org
Foci were quantified by manual counting and the Cell Profiler Software (www.cellprofiler.org).org
Images were analysed using Cell Profiler [27] to identify DAPI labelled nuclei by Otsu thresholding, and measure the intensity of OCT4 and NANOG immunolabelling in the detected areas.
Cell profiler software [17] allows users to combine image-processing methods to develop techniques to count cells, but this requires some knowledge of computation and it has not been tested for specific applications.
Nuclei were counted with Cell Profiler (Carpenter et al., 2006).
The Cell Profiler pipeline developed for this study is provided as a.cp file (Supplementary file 1).
Similar(29)
GWB were counted using Cell-Profiler image analysis software [ 25]. siRNAs targeting TRAF6 and IRAK-1 were transfected into THP-1 cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), in accordance with the manufacturer's instructions.
mESC and MEF samples were analysed using the Mouse Embryonic Stem Cell RT2 Profiler PCR Array (SABiosciences).
Human Stem Cell RT2 Profiler™ PCR Array (PAHS-405A, SABioscience, USA) was done according to the manufacturer's instructions.
To further explore the biological properties of liver CSCs, the Stem Cell RT2 Profiler™ PCR array was performed.
A pluripotent stem cell protein profiler array kit (R&D Systems, Oxfordshire, UK) was used to further determine treatment-induced effects on the stem cell compartment.
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