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At day 3.5 post IR, a striking reversed pattern was observed in WT animals, with a significant increase in the frequencies of PCNA-positive cells at cell positions 1 to 19.
Differences were deemed as significant when P < 0.05 by ANOVA and when cell positional distributions were different at three or more consecutive cell positions (27).
This was in sharp contrast to a significant reduction in the number of PCNA-positive cells at the majority of crypt cell positions in Rad21+/− mice (Fig. 6B).
In the trunk, EGFP-positive cells could assume tip or stalk cell positions, indicating that transgenic dll4 expression was globally compatible with angiogenic behaviors.
At day 1 post IR, the frequency of PCNA-positive cells was dramatically reduced to 2 3% at these cell positions in irradiated WT colonic crypts (Fig. 6B).
Strikingly, the frequencies of PCNA-positive cells in irradiated Rad21+/− animals were significantly higher at all cell positions compared to that of irradiated WT (Fig. 6B).
I have predefined cell positions in hdf5.
The cell nucleus is fluorescently labelled to allow automated tracking of cell positions.
Cell nuclei are labelled for semi-automated detection of cell positions.
Is there a possibility to export cell positions from hdf5 and set it in the model?
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BrdU-positive cell position and number were scored.
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