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For each strain the number of granules per full size cell picture (ngranules) was determined as well as the total number of full length cells (ncells, or n in formulas 5 and 6).
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I was very honest with them, like, 'I'm new to this.' " Mr. Stern mentioned a dance remix of "God Only Knows," the classic Beach Boys track Ms. Moore covers in the film, which was being sent to clubs with backing vocals by his production partner in Single Cell Pictures, Michael Stipe, the R.E.M. singer.
Each cell pictured by rectangle created by rectangle exhibits a proportion of the particular category for the particular variable.
Cell pictures were captured with METAMORPH V5.0 (Universal Imaging, Media, PA, USA).
Bright field and fluorescent live cell pictures were taken on an Axiovert 200 microscope (Zeiss) using a Qicam fast camera (Qimaging) and the Openlab 4.0.4 software.
Cell pictures were acquired every 5 min using the MetaVue software.
In this assay cells were plated at ~30 50% confluence depending on the growth rate of each cell and to keep control untreated cells viable (see cell pictures in Supplementary Figure 1).
HCF-1 silencing depletion by siRNA treatment, HCF-1 (Rhodamine) and BrdU (FITC) staining for determination of endogenous HCF-1 depletion and cell-cycle progression analysis, respectively, and fluorescent-cell picture acquisition were as described [6].
A small nucleus is present at the centre of some cells (picture not shown).
In general, NOA patients had a kind of mosaic Leydig cells picture across the same bioptic sample: in some areas Leydig cells had a regular appearance; in other areas, their morphology has been changed– 1(d)).
Rather than clone humans, researchers take the early stage embryos that result from SCNT and then derive stem cells (pictured above, fluorescently tagged red).
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