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In our model, stem cells were placed into an array of 50 x 20 cell patches, with each patch carrying a maximum of 64 cells.
Cell patches are widely used for healing injuries on the surfaces or interfaces of tissues such as those of epidermis and myocardium.
Here we report a novel type of porous scaffolds made of poly d,l-lactic-co-glycolic acid) for fabricating cell patches.
Cell patches with intact cell cell and cell extra cellular matrix contact attached and adhered on a scaffold within 1 h.
Thus, our study concludes that multiscale patterned transplantable stem cell patches may have a great potential for bone regeneration as well as for various regenerative medicine approaches.
These results demonstrate the feasibility of utilizing biomimetic substrates not only as platforms for studying the influences of the ECM on skeletal muscle function and maturation, but also to create transplantable muscle cell patches for the treatment of chronic and acute muscle diseases or injuries.
Similar(32)
: (whole cell patch clamp) of Huguenard & Prince, J. Neurosci.
: (whole cell patch clamp) of Huguenard & Prince, J Neurosci.
This led to the disintegration of the cell patch (Fig. 7a, right panel, 48 h).
Whole cell patch-clamp recordings were made to analyze the ICa, and [Ca2+]i was measured with fura-2.
Whole cell patch clamp recording was performed in primary cultured rat trigeminal ganglionic neurons.
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