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Jackson SJ, Singletary KW. "Sulforaphane inhibits human mcf-7 mammary cancer cell mitotic progression and tubulin polymerization".
A promising alternative to eliminate cancer cell mitotic progression relies on interference in the function of some essential mitosis molecules such as microtubule-associated proteins, particularly spindle motor proteins [ 6, 7].
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Similar results were obtained in three independent experiments indicating that in LASP-1-silenced SKOV-3 cells mitotic progression cannot proceed normally.
We also observed increased number of multinucleated cells and mitotic progression delay in CUL3 depleted cells compared to control cells (data not shown, Fig. 1C) suggesting CUL3 has functions in mitotic progression and cytokinesis, consistent with previous reports (Sumara et al., 2007; Maerki et al., 2009).
CENP-H is considered to be crucial for cell growth and mitotic progression [14].
Aur is essential for several aspects of the cell cycle and mitotic progression (Berdnik and Knoblich 2002; Marumoto et al. 2003; Portier et al. 2007).
To clarify the role of β-tubulin isotypes in mitosis, we studied how changes in their expression affect cell morphology and mitotic progression.
These deletion strains are typically related to cell cycle and/or mitotic progression (e.g., SPC42, G. Brown and C. Nislow, unpublished results).
CENP-H is essential for cell growth and mitotic progression [14].Since our previous data indicated that CENP-H may be a marker of cancer development [19], [20], our goal was to analyze the relationship between CENP-H and Ki-67, one of the most frequently used markers of proliferating cells.
In contrast, targeting of chromosome tips during anaphase resulted in a significant proportion of cells with perturbed mitotic progression (61%, N = 94/132 cells).
Finally, we examined the effects of transfecting cells with non-phosphorylatable mutant forms of cdc25C into U2OS cells and scoring mitotic progression.
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