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Mouse EpiSC are poorly compatible with embryo chimerism, at least by standard ES cell methods of blastocyst injection and morula aggregation, but retain the ability to differentiate to multiple tissues as judged by teratoma formation and in vitro differentiation [10], [11].
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The most appropriate scanning exposure time was selected by choosing the datasets with the least number of saturated spots (scanning intensity > 65,000) and the highest number of spots above the average background (cell method of background calculation).
TA was analyzed using the open-cell method of potentiometric titration at 25 °C with a precision of ± 0.1%59,60.11.
Preferential misincorporation of G versus A opposite the T template is detectable by gel electrophoresis in crude extracts of animal cells (Method of Gening, abbreviated here as misGvA [31]; [31]).
Information about the date of collection, number of isolated cells, method of DNA extraction and primer combination for each DNA sequence is shown in Table 4 [GenBank accession codes FJ947036– FJ947036].
Diversity in the source of original cell populations, methods of isolation, and preparation of cells could affect cell fate and the resultant effect of the cells on the host microenvironment.
The study of this relationship may benefit tremendously from cell reprogramming methods, of which there are three.
The stem cell method is also capable of growing many more cells from a smaller starting batch than the biopsy method.
In fact, the methods for cell isolation and purification, the procedures for their storage or propagation, the phenotype and number of injected cells, and the methods of cell administration are hugely inconsistent between studies.
With the loss of motile cells, alternative methods of gamete release and dissemination evolved in fungi.
"Our stem cell method provides an unlimited source of cells from the patient for transplantation and isn't limited by the availability of existing hair follicles".
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