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Cell lysis buffer and polymerase chain reaction (PCR) kits were purchased from Tiangen, China.
The 5-amino-4-imidazolecarboxamide ribonucleotide (AICAR) and cell lysis buffer were from Beyotime (Shanghai, China).
The cells were lysed in cell lysis buffer containing PMSF for 30 min at 4°C.
Cells were washed with 2 mL PBS, lysed with 150 μL cell lysis buffer on ice for 2 min.
1 mL cell lysis buffer and 1 mL acid-washed glass beads (D = 0.5 mm) were added.
Protein was harvested using Cell Lysis Buffer from Cell Signaling.
Protein was extracted by RIPA cell lysis buffer.
The cells were harvested 24 hours later and lysed using passive cell lysis buffer (Promega).
Cells were harvested and lysed in cell lysis buffer as previously described above.
Contaminating erythrocytes were eliminated from the SVF by incubation in red blood cell lysis buffer (Sigma).
Total protein was isolated from U251 and U87 transfected cells in mRIPA cell lysis buffer.
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