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Whole cell lysis and immunoblotting were performed as described63,68.
The mechanism of lead-dependent cell lysis was explored next.
For complete red blood cell lysis, ACK treatment was repeated.
It is often assumed that exit of non-enveloped viruses such as poliovirus requires cell lysis.
Increased expression of PD-L1 results in increased resistance to NK cell lysis.
Association of Argonaute proteins and microRNAs can occur after cell lysis.
Following staining with DAPI, cell lysis was assessed using flow cytometry.
Briefly, tumor cells were isolated from ascites after centrifugation and red blood cell lysis.
Of note, our ECM extraction procedure did not cause cell lysis during experiments17.
A gentle cell lysis protocol was used to avoid damage to the expressed polyproteins48.
Cell suspensions were passed three times through an EmulsiFlex-C5 to facilitate cell lysis.
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