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The pFBD/KCT stable transfection significantly enhanced the cytotoxicity of 5′-DFUR to both RKO and H630 cell lines (over 28-fold).
We tested switch activation in cell lines over a wide range of telomerase promoter activities.
AANTs exhibited excellent biocompatibility in both cell lines over a time course of five days even at a maximum concentration of AANTs of 100 μg mL−1.
Among them, compounds 9d, 10b, 10d and 11g with good antitumor activities exhibited high selectivity for tumor cell lines over immortalized mouse hippocampal (HT22) cell line.
The scientist aims to create hundreds or thousands of cell lines over the next few years as part of the Personal Genome Project, an effort that he launched two years ago to capitalize on advances in gene-sequencing technologies.
Currently, in vitro approaches typically range from single organ assays e.g.: the isolated whole heart from preclinical species, isolated ventricular cardiomyocytes from preclinical species to cell lines over expressing single ion channels13,14,15.
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Finally, we generated stably transduced mpkCCDc14 cell lines over-expressing miR-466g.
In addition, the new derivatives were also active on multidrug-resistant cell lines over-expressing P-glycoprotein.
To demonstrate the role of collectrin in cilia formation, stable mIMCD3 cell lines over-expressing collectrin (collectrin stable) were generated.
We generated stable inducible Tet off SH-SY5Y cell lines over-expressing human A53T ASYN or the ΔDQ/A53T ASYN that lacks the CMA recognition motif.
Compared to the controls, cell lines over-expressing miR-30a or -191 do not show significant changes in the cell cycle distribution (Figure 3B).
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