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The cell lifecycle phase durations are marked on the figure.
The cell lifecycle is composed of two main periods called interphase and mitosis [ 24].
These initial results show the potential of the parameters to distinguish between the different phases of the cell lifecycle, as well others biological phenomena.
We can elaborate ISEA to include higher granularity components and mechanisms that map to subcellular details such as cell lifecycle pathways and intercellular signaling networks when validation against an expanded set of targeted attributes requires doing so.
We conclude that the cell in the beginning of the experiment is at G1 phase by knowing that we started the experiment right after cell division (the way we determine the rest of the cell lifecycle phases will be explained later).
Using these phase-based parameters, we investigated the life cycle of ten HeLa (human cervical cancer) cells, and showed initial abilities of the parameters to distinguish between the different stages of the cell lifecycle, predict upcoming lifecycle phases, and measure biologically useful properties.
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Those results meet our expectations that during most of the HeLa cells lifecycle, until the mitosis phase, the cells are flatten (this will also be confirmed later in Figs. 7 and 9, presenting the cell at phases S, G2 and M).
Large molecular complexes, formed by interacting proteins, perform numerous biological processes vital to the cell's lifecycle.
Previous works used IPM to characterize growth of cells with a short lifecycle, such as yeast cells [ 19] and other eukaryotic cells, using their dry mass and morphological changes [ 4, 20].
HeLa cells have a lifecycle duration that can last for up to 30 hours, which requires long and continued periods of imaging.
Since these cells have a lifecycle that can last up to 30 hours, we constructed an IPM setup with an integrated temperature control that is capable of imaging the quantitative phase profiles of the cells for more than 48 hours.
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