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Ebner, P. et al. Non-classical protein excretion is boosted by PSMα-induced cell leakage.
Study suggests that these grooves facilitate the convective medium transport throughout the cryogel beads in order to eliminate the substrate and product inhibition and also prevent cell leakage.
According to our presented in vitro investigations, the GS immunoconstruct exhibited effective elimination of carcinoma cells as well as high safety free of gene alteration or cell leakage.
Partial necrosis due to cell leakage, apoptosis due to calcium influx, cell membrane reversible and irreversible electroporation, oxidative damage to the membrane, local pH changes, changes caused by reactive oxygen species, and other mechanisms have been proposed in the last four decades and are currently under scrutiny16.
We demonstrated that inclusion of alginate gel in both designs is efficient in preventing cell leakage to the surrounding yet permitting the GDNF secretion, although the cellular growth rate is reduced in an alginate concentration dependent manner.
All the surfaces of the collagen sponge except the top were wrapped with a membrane that has pores smaller than the cells to protect against cell leakage during cell seeding.
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Fig. 4 Profiles for Daptomycin production using different mode of cells and cell leakage-free cells, pelletized cells, immobilized cells on refractory brick and immobilized cells on silk sachets.
Type-III rejection started with ampulla tips' rupture and blood cells leakage through the shattered epithelium.
Histopathologically, the pneumonia in Case 1 was severe with the presence of inflammatory cells, leakage of blood into the lung tissue, and intralesional gram positive cocci, which were likely to be S. equi subsp. zooepidemicus.
Accurate intracellular metabolite quantification requires sampling procedures that rapidly stop metabolism (quenching) and avoid metabolite loss via the cell membrane (leakage).
The results suggest that cell membrane leakage was dose dependent and significantly affected (Figure 12).
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