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It is particularly noteworthy that Ek was highest when photons were most abundant (Figure 3C), indicating that the photosynthetic machinery of the cell is running near its maximal capacity (i.e. Pcell/Pcellmax≈1) for a large portion of the day (Figure 3D), even though optimal light utilization efficiency (αcellmax) may not be achieved.
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The flow cell was run for 48 h using the standard MinKNOW software (version 1.11.5) and reads generated were base-called in real-time using the cloud-based Metrichor service provided by Oxford Nanopore Technologies.
A NTA flow cell was run in parallel in the same conditions as a blank.
The flow cell was run for 6 hours for each sequencing run.
The Illumina GAIIx flow cell was run for 75 cycles using a single-read recipe (v4 sequencing kits) according to the manufacturer's instructions.
The cell was run for varying times, Vancomycin concentrations and voltages, to gain information on optimisation of conditions for impregnating the graft.
The flow cell was run for 76 cycles of sequencing on an Illumina GAIIx sequencer with an additional six cycles for the indexing.
For fruit flies, the fountain of youth may be filled with an energy-sensing protein usually activated when cells are running out of steam.
Thus, PTEN mutant cells are running at the edge of survival under limited nutrient conditions.
Furthermore comparison of lane 3 and 5 also shows that the non-glycosylated rhDNase from T. thermophila and CHO-cells are running at heights of comparable molecular weight.
All the four EIX cells were run for a maximum of 7 h.
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