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In a paper published Thursday in the journal Cell, investigators from Harvard and MIT show that this technique can be used to manufacture antimicrobial compounds, vaccines, and antibodies that are just as effective as traditionally made drugs.
So far, in research that stem cell investigators say is among the most promising in the field, Dr. Zon and Dr. Daley have turned mouse embryonic stem cells into mouse blood cells.
And speaking of partnerships, Beckett expects that the network and the Centre for Stem Cell Research will collaborate, with the latter providing the physical space and common facilities for stem cell investigators who belong to both organizations.
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High-content image analysis software (IN Cell Investigator) is also integrated into this platform.
While patients and other models have yielded important clinical insights into this disease, we were particularly interested in its molecular aspects—how specific genes can affect responses to glucose by the beta cell," said co-senior author Dieter Egli, PhD, Senior Research Fellow at NYSCF, who was named a NYSCF Robertson Stem Cell Investigator in 2012.
Image analysis was performed using IN Cell Analyzer Workstation 3.5 software (IN Cell Investigator, GE Healthcare) and a Dual Area Object Analysis algorithm as previously described [31].
The hydroethidine signal was quantified using the In Cell Investigator 3.4 software.
Images were quantified with ImageJ (Fiji version), IN Cell Investigator, CellProfiler, Zeiss Zen, and Adobe Photoshop.
Cells were imaged using the In Cell Analyzer 1000 and pictures were analyzed using the In Cell Investigator 3.4 software.
Cells stained on 96-well plates were imaged using the In Cell Analyzer 1000 (GE Healthcare) and signal measurements and statistics were performed by the In Cell Investigator 3.4 software (GE Healthcare).
For quantification of cell cultures (triplicate wells per condition), images were captured using either the Leica DMI4000B epifluorescent microscope (20X objective) and analyzed using ImageJ (NIH) software or the GE IN Cell Analyzer 2000 (20X or 10X objective) and analyzed with the GE IN Cell Investigator software suite.
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