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All experiments using live cells were performed in a customized live cell chamber (Live Cell Instrument, South Korea).
Dishes were kept warm during imaging using a Chamlide TC-L live-cell environment (Live Cell Instrument).
To maintain the standard tissue culture condition for ECs in the flow chamber, we placed all the components, except the syringe pump, in a chamber (Chamlide TC, Live Cell Instrument, Korea) in which the temperature and CO2 concentration were controlled.
To maintain the standard tissue-culture conditions (37°C, 5% CO2) in the flow chamber for ECs, we placed the components, except the syringe pump, in the temperature and CO2 control chamber (Chamlide TC, Live Cell Instrument, Korea).
Imaging was carried out using an OLYMPUS inverted microscope with a 100×/1.49 numerical aperture TIRF objective, equipped with through-the-objective TIRF illumination, a 37 °C temperature-controlled stage and an objective warmer (Live Cell Instrument, Seoul, Korea).
All of the experiments were conducted on the stage of an inverted microscope (TE2000-U, Nikon, Japan) with an installed microscope incubation system (Chamlide TC, Live Cell Instrument, South Korea).
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Specimens are systematically studied using an oedometeric cell instrumented to study the evolution of small strain stiffness, electromagnetic properties, and volume change.
Cells were assembled in an argon-filled glove box, and the electrochemical test of discharge-charge properties and cyclic voltammetric were tested on the CT2001A cell test instrument (Wuhan LAND Electronic Co., Ltd) and CHI660E (Shanghai Chenhua instrument Co., Ltd) electrochemical workstation, respectively.
These CRC nucleated population have been linked to cell-to-cell and cell to instrument interactions that are limiting factors in terms of recovery and viability.
At least 10,000 events were measured using a fluorescence-activated cell sorting instrument (FACSCalibur; Becton Dickinson, San Jose, CA, USA), and cell flow cytometry data were analyzed using CELLQUEST software (Becton Dickinson).
We used the cell flow instrument to detect the level of Lymphocytes and subsets and immunoglobulin and complement in the peripheral blood, and by ELISA detecting the content of cytokines (IL-17, IL-4, IL-5, TNF-α, INF-γ) in the serum of pituitary adenoma patients.
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