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Whereas the upside morphology of the cell could be easily obtained by atomic force microscopy (AFM) after cell fixation, the downside morphology of the same cell could be retrieved after reversed cell imprinting.
We note that it is possible that disruption of Dazl may directly or indirectly impact imprinting status; previously, germ cell specific genes, other than components of the cellular enzymatic machinery such as the demethyltransferase family, have not been shown to impact germ cell imprinting.
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Briefly, post differentiation patterning suggests that β-catenin signalling acts on proliferating or differentiating cells imprinting them to express GS and rendering this expression to be regulated by various other signals.
Gene expression is the nervous system of cells, imprinted by anything in the environment.
A significant underestimation of antigen-experienced Th cells imprinted for expression of distinct cytokines is the consequence, as demonstrated here for Th cells isolated ex vivo from inflamed tissue or secondary lymphoid organs of mice with colitis.
Th1 and Th17 cells are distinct lineages of proinflammatory effector/memory cells, imprinted for reexpression of interferon-γ (IFN-γ) and interleukin-17 (IL-17), by upregulated expression of the transcription factors T-bet and RORγt, respectively.
To determine whether the correct cycle of germ cell imprint erasure and re-establishment occurs in vitro in the absence of Dazl, we examined methylation at the H19 DMR in germ cells differentiated from male wildtype and Dazl-null mESC lines.
Briefly, cell imprints were prepared by applying a freshly cut surface of the tumour on glass slide.
DNA content was determined by image cytometry on cell imprints, and Cdc20 and securin immunohistochemistry on tissue microarrays of breast cancer tissue.
The histological diagnosis of all cell imprints was morphologically verified from routine formalin-fixed (pH 7.0), paraffin-embedded and H&E-stained tissue specimen from the same cut surface of the tumour.
A study by Chang et al which demonstrates that effector and memory T cell lineage imprinting can occur as early as the first cell division underscores the need for understanding the determinants of CD8+ T response during the early phase of the response [13].
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