With the increasing availability of live cell imaging technology, tracking cells and other moving objects in live cell videos has become a major challenge for bioimage informatics.
The recent advancement of live-cell imaging technologies has provided a variety of powerful tools and allowed the collection of a vast amount of image data/movies which requires significant time and effort on image analysis and quantification.
By using different live-cell imaging technologies that allow us to follow the position of ARTD10 molecules over time, we found that ARTD10 shuttles constantly in and out of the nucleus.
Furthermore, virtually all stem cells imaging technologies require some degree of ex vivo alteration of the stem cells prior to implantation of the cells, and so efficiency of alteration is also a requirement.
The work by Tung and his colleagues is part of a larger effort in the cancer research community to use nanoparticles to detect the presence and measure the activity of tumor cells through imaging technologies.
Images were taken using EVOS XL Core cell imaging system (Life technologies).
The advent of live cell imaging and GFP-labelling technologies in the 1990s (Tsien, 1998) have greatly facilitated the study of proteasome dynamics in yeast and mammalian cells.
Finally, the possibility of combining this cell patterning technique with high definition imaging technologies opens the door to a new generation of single cell studies: the behaviour of single cells (spreading, polarisation, differentiation, division) may be controlled using our micro-patterns and probed using a combination of live imaging and FRET.
The purpose of this Review is to highlight the unique strengths of zebrafish cancer models in assessing the roles that intratumoral heterogeneity and clonal evolution play in cancer, including transgenesis, imaging technologies, high-throughput cell transplantation approaches and in vivo single-cell functional assays.
